BRAF Mutation Kit

Transitional cell carcinoma is the most common cancer of the urinary tract in the dog. The most common methods of diagnosis are urinary cytology, suction biopsy of the bladder, and tissue biopsy via cystoscopy or surgery. All of these methods detect cancer late in the course of the disease.

The CADETSM BRAF Mutation Detection Assay is a urine-based DNA test for early detection of canine transitional cell carcinoma. The BRAF mutation is found in 85% of canine transitional cell carcinoma cases and has not been detected in the urine of dogs with other cancers nor in dogs with non-neoplastic inflammatory bladder disease or nonmalignant bladder masses. The test has been shown to detect transitional cell carcinoma in dog up to 4 months prior to the development of clinical symptoms. The BRAF Mutation Detection Assay is not affected by hematuria or bacteriuria.

Dogs that test positive for the mutation can be monitored during treatment to detect the extent of residual disease.

Each kit contains everything needed to collect and submit the sample. There is a small cardboard box and a white envelope for each kit.

Prepare the urine collection pot

  1. Remove collection pot from the clear plastic bag and discard the bag.
  2. There is a small volume of clear liquid preservative in the pot. DO NOT DISCARD the liquid.
  3. Label the collection pot with the first and last name of the dog in CAPITAL LETTERS

Sample collection

  1. Collect urine by free catch or catheter into a separate clean container.
  2. 40-60ml of urine is required for each test
  3. If the volume cannot be collected at once, it is ok to collect smaller volumes over 2-3 days.

Packaging and Shipping

  1. Screw the top on tightly and place collection pot inside the supplied bag with absorbent pad.
  2. Package bag inside small cardboard box provided. Place box inside supplied FedEx bag.
  3. Complete green Sample Submission Form.
  4. Ship via FedEx. NOTE – cannot be shipped using FedEx drop box
  5. Send an email to CADETINBOUND@sentinelbiomedical.com with collection pot number (Dx) and FedEx tracking number in the subject header.

Optional Study Participation

The bottom half of the green submission form is a consent form and questionnaire for an optional study investigating genetic and environmental factors associated with canine TCC.

Test results are emailed within 2-3 business days of receipt of the sample at Sentinel Biomedical.

Additional resources

  • PDF version of this page
  • CADET SM BRAF Mutation Detection Assay Brochure from Sentinel Biomedical. Read brochure
  • Mochizuki H, Shapiro SG, Breen M. Detection of BRAF Mutation in Urine DNA as a Molecular Diagnostic for Canine Urothelial and Prostatic Carcinoma. PLoS One. 2015;10(12):e0144170. doi: 10.1371/journal.pone.0144170. PubMed PMID: 26649430. Download article
  • Decker B, Parker HG, Dhawan D, Kwon EM, Karlins E, Davis BW, et al. Homologous Mutation to Human BRAF V600E Is Common in Naturally Occurring Canine Bladder Cancer–Evidence for a Relevant Model System and Urine-Based Diagnostic Test. Mol Cancer Res. 2015;13(6):993-1002. doi: 10.1158/1541-7786.MCR-14-0689. PubMed PMID: 25767210; PubMed Central PMCID: PMC4470794. Download article
Last updated: 2018-01-19